FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
发明人:
DIMITRIADIS, Nikolas,DELIOLANIS, Nikolaos
申请号：
EPEP2015/062448
公开号：
WO2015/185662A3
申请日:
2015.06.03
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
The present invention relates to the multispectral imaging of samples, in particular of biological tissues. The invention further relates to a method for acquisition of fluorescence images and reflection images of an object (400) comprising the steps of alternatingly illuminating the object (400) with at least a first light and a second light, wherein the first light and the second light are spectrally shaped such that at least one light has several spectral regions of high light intensity separated by spectral region(s) of low light intensity, wherein the spectral regions of the first light and the second light with high intensity at least partially do not overlap and wherein at least one of the two lights has at least one region of low light intensity that is of longer wavelength to the neighboring region of high light intensity, and recording at least a first image of the object (400) and a second image of the object (400) while illuminating the object (400) with at least one of the lights wherein the light to be recorded as the first image is modified such, that at least one spectral region of high intensity of the second light is attenuated, and wherein the light to be recorded as the second image is modified such, that at least one spectral region of high intensity of the first light is attenuated.La présente invention concerne l'imagerie multispectrale d'échantillons, en particulier de tissus biologiques. L'invention concerne également un procédé d'acquisition d'images de fluorescence et de réflexion d'un objet (400) comprenant les étapes consistant à éclairer alternativement l'objet (400) à l'aide d'au moins une première lumière et d'une seconde lumière, la première lumière et la seconde lumière étant spectralement telles à ce qu'au moins une lumière comprenne plusieurs régions spectrales de haute intensité lumineuse séparées par une ou plusieurs régions spectrales de faible intensité lumineuse, les régions spectrales de la première lumière et de la seconde
