Provided are methods and devices for evaluating dynamic middle ear muscle activity in a subject. A probe is provided having a speaker and a microphone in sound-wave communication with an eardrum associated with the middle ear muscle of the subject. A sound wave is generated from the speaker and transmitted to the eardrum. The sound wave that is reflected is detected and a reflected sound wave property measured. The input sound wave may be comb input to fully extend ossicle movement in all available vibratory modes, thereby providing maximum information as to dynamic middle ear muscle activity.
