PROBLEM TO BE SOLVED: To provide production methods of Poaceae plant with cytoplasmic male sterility, and to provide Poaceae plant with cytoplasmic male sterility.SOLUTION: The present invention provides a protein encoded by the cytoplasmic function regulating gene of a nuclear gene, in a Poaceae plant, and a method for producing a Poaceae plant with cytoplasmic male sterility by deleting or reducing the function of a protein selected from: (1) a protein having an amino acid sequence represented by a particular sequence (2) a protein which has an amino acid sequence having 70% or more sequence identity to an amino acid sequence represented by a particular sequence, and has a function localizing in mitochondria and (3) a protein encoded by the ortholog of a gene encoding a protein having an amino acid sequence represented by a particular sequence.SELECTED DRAWING: Figure 6COPYRIGHT: (C)2019,JPO&INPIT【課題】細胞質雄性不稔性イネ科植物の作製方法及び細胞質雄性不稔性イネ科植物の提供。【解決手段】イネ科植物において、核遺伝子の細胞質機能制御遺伝子がコードするタンパク質であって、以下から選択されるタンパク質の機能を喪失又は低減させることにより、細胞質雄性不稔性イネ科植物を作製する方法。(1)特定の配列で示されるアミノ酸配列を有するタンパク質。(2)特定の配列で示されるアミノ酸配列と70%以上の配列同一性を有するアミノ酸配列を有し、ミトコンドリアに局在する機能を有するタンパク質。(3)特定の配列で示されるアミノ酸配列を有するタンパク質をコードする遺伝子のオーソローグによりコードされるタンパク質。【選択図】図6
