A kind of water steams the efficacy testing method of Shi Yan Smoked agent,Test bacterium is mainly diluted to different dilution bacterium solutions Hou with sterile diluent,To no Yan Smoked agent control group Ji Yan Smoked agent test group carries out pumping filtering and draws bacterium solution,Make on bacterium uniformly dispersing what filter membrane,It will be on the smooth what water agar medium of filter membrane； Hou is put into Yan Smoked agent to the culture medium opening and filter membrane that test group is posted filter membrane upward,When no longer San Chu Smoked cigarette,Ji Shi Smoked steams the time,Control group is equally that culture medium is opened and the filter membrane direct time synchronisation of Hou upward； Smoked steams the time once to starting suction motor,Cigarette extraction Hou is taken out to the water agar medium of test group and control group,Filter membrane is converted to Tryptose soy agar medium,Bacterium number is calculated via program Hou is cultivated. The water that bactericidal agent is solid can so be tested and steam Shi Yan Smoked insecticide, traditional test mode is solved and be only capable of applying the missing of what liquor.一種水蒸式煙燻劑的藥效測試方法，主要是將測試菌以無菌稀釋液稀釋成不同稀釋度菌液後，對無煙燻劑對照組及煙燻劑試驗組進行抽氣過濾並吸取菌液，使菌均勻散布於濾膜上，將濾膜平貼於水洋菜培養基上；把試驗組貼有濾膜的培養基打開且濾膜朝上後放入煙燻劑，當不再散出燻煙時，計時燻蒸時間，對照組同樣是培養基打開且濾膜朝上後直接同步計時；燻蒸時間一到就開動抽氣馬達，將煙抽出後取出試驗組與對照組的水洋菜培養基，把濾膜轉換至胰蛋白大豆瓊脂培養基上，經由培育程序後計算菌數。如此即可測試滅菌劑為固型物之水蒸式煙燻殺蟲劑，解決傳統測試方式僅能應用於液劑的缺失。
