[Object] Proposed is a new and improved illuminating device, a control method for the illuminating device, and an image acquisition system which can adjust light amounts of a plurality of respective light sources on the basis of correlations between a luminance acquired from a light receiving unit and the light amounts of the emission light beams so as to precisely adjust the color temperature in the light receiving unit.[Solution] An illuminating device includes: a white light source configured to emit white light; RGB light sources light amounts of respective emission light beams of which are independently adjustable; a multiplexing unit configured to multiplex the white light and the emission light beams into illumination light; and a control unit configured to control the light amounts of the respective emission light beams.