FLANDEZ, JEANNE GRACE,IRVING, BRYAN A.,LA PORTE, SHERRY L.,BOUSTANY, LEILA MARIE
申请号：
ARP180102968
公开号：
AR113446A1
申请日:
2018.10.12
申请国别(地区):
AR
年份:
2020
代理人:
摘要：
1. This contract provides antibody and active antibody (AA),2. Bispecific antibody and bispecific active antibody (BAA). Methods for preparing and using these antibodies, AA, BIAB and baa, are also provided. Target 1: a double active antibody (BAA),a. When baa is enabled, it specifically binds to two targets； this is an active dual-purpose antibody, which is characterized by the following structure: (a) Ab1 antibody specifically binds to the first target, Ab1 includes two heavy chains (Ab1 HCS) and two light chains (Ab1 LCS)；Ab1 is combined with the first masked allowance (MM1) and the first separable allowance (CM1) to form an mm1-cm1 building complex, where the final carbohydrate end of the mm1-cm1 building complex is connected to each amino end of each light chain of Ab1, wherein: MM1 inhibits the combination of Ab1 and its target； CM1 is a polypeptide that acts as a matrix for the first protein, (b) two scFvs (each AB2) specifically bind to the second target, each AB2 containing a variable light chain region connected to a variable heavy chain region,There, the terminal carboxilo end of each AB2 is connected to the terminal Amin end of each heavy chain of Ab1； there, each AB2 is combined with the second mask margin (mm2) and the second separable margin (cm2) to form an mm2-cm2 structure, Where the final carbohydrate end of each mm2-cm2 building is connected to the Amin end of each AB2, where mm2 inhibits the binding of AB2 to the target, while cm2 is a polypeptide that acts as a matrix for the second protein, Moreover, baa has at least one of the following characteristics: (1) mm2 includes the amino acid sequence in SEQ ID 12；MM1 includes the amino acid sequence selected from the group, which is composed of the sequence listed in Table 7； AB2 includes the heavy chain variable field as shown in SEQ ID 2 or SEQ ID 3 or the light chain variable field as shown in SEQ ID 1 or SEQ ID 4； Ab1 includes: An Fc region, including at least one amino acid substitute at l234,
