The present invention provides the gene therapy method of improvement and compositions. In a particular embodiment, gene therapy includes the candidate stem cell and progenitor cells composition and its making and use method of the curative effect with increasing. In other specific embodiments, it is contemplated by the invention that the composition and method of transduction efficiency and vector copies (VCN) for increasing mankind hemopoietic stem cell and progenitor cells (HSPC), to generate the gene therapy compositions of improvement. In various embodiments, the HSPC groups transduceed through slow virus carrier are imagined in part of the present invention. In various embodiments, it is contemplated by the invention that the method for the drepanocytosis for the treatment of subject, the method includes applying a effective amount of hematopoietic cell population contemplated herein to the subject. In various embodiments, it is contemplated by the invention that a kind of kit of reagent and staurosporin (staurosporine) including increasing the conduction of prostaglandin E P receptor signals.
