Disclosed is a standardised aqueous biorelevant media for simulating fasted state stomach and fasted state upper small intestinal fluids of mammalian species, composed of surfactants occurring in the gastro intestinal tract of mammals comprising (a) at least 40 mole % to 95 mole % of one bile salt, and (b) the rest mole % being a combination of at least two surfactants, namely a diacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a monoacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a diacyl phospholipid and a monoacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a diacyl phospholipid and a monoacyl phospholipid, which further is defined by a surface tension between 25 mN/m and 50 mN/m. Disclosed is further a homogeneous biorelevant composition for preparing fasted state biorelevant media characterised by a surface tension between 25 mN/m and 50 mN/m comprising the following surfactants: (a) at least 40 mole % to 95 mole % of one bile salt, and (b) the rest mole % being a combination of at least two surfactants, namely a diacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a monoacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a diacyl phospholipid and a monoacyl phospholipid and a fatty acid including monovalent salts of the fatty acid, or a diacyl phospholipid and a monoacyl phospholipid.Cette demande concerne une composition homogène biologiquement pertinente pour préparer un milieu biologiquement pertinent de l'état de jeun ayant une tension de surface entre 25 mN/m et 50 mN/m pour simuler le jeun gastrique et le jeun sur les liquides de l'intestin grêle d'espèces mammaliennes, en particulier les êtres humains, les chiens, etc. comprenant au moins un sel biliaire, par exemple du taurocholate de sodium ou du taurodésoxycholate de sodium ； au moins un phospholipide, en particulier 60-99% en poi
