СТАГЛИАНО Нэнси Э. (US),УЭСТ Джеймс У. (US),КАМАТ Катрин (US),БЕССЕТТ Пол Х. (US),СЭЙДЖЕРТ Джейсон (US)
申请号：
RU2011138951/10
公开号：
RU2011138951A
申请日:
2010.02.23
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. An activated polypeptide containing a functional protein or fragment of a functional protein that is not an antibody or fragment of an antibody, where the functional protein is connected to a peptide mask that: (i) inhibits the binding of a functional protein or fragment of a functional protein to a binding partner and (ii) does not has the amino acid sequence of a binding partner, and where the functional protein or fragment of the functional protein is additionally connected to a cleavable linker capable of cleaving such azom that: (i) in the unsplit state mask peptide inhibits the binding of a functional protein or functional protein fragment with a binding partner, and (ii) a cleaved peptide mask is not able to inhibit the binding of a functional protein or functional protein fragment with a binding partnerom.2. The activated polypeptide of claim 1, wherein the functional protein or functional protein fragment has an equilibrium dissociation constant of not more than 100 nM for binding to a binding partner； the peptide mask (A) has an equilibrium dissociation constant for binding to a functional protein or functional protein fragment greater than the equilibrium dissociation constant of a functional protein or a fragment of a functional protein with a binding partner, (B) contains a polypeptide sequence that is no more than 50 % is similar to the natural binding partner of a functional protein or functional protein fragment, and (C) does not prevent the functional protein or functional protein fragment from binding to the binding partner1. Активируемый полипептид, содержащий функциональный белок или фрагмент функционального белка, которые не являются антителом или фрагментом антитела, где функциональный белок соединен с пептидной маской, которая: (i) ингибирует связывание функционального белка или фрагмента функционального белка со связывающим партнером и (ii) не имеет аминокислотной последовательности связывающего партнера, и где функциональный белок или фра
