INVENTION : BIOTECHNOLGGICAL HAIR SHAMPOO ABSTRACT: One of the hygienic practices, is to use shampoos and soaps for hair cleaning, deodouring and reducing micro organisms to avoid the hair diseases Hke dandruff. Commercial shampoos are having detergents, shampoo base, Triclosan, Isopropyl, preservatives, thickening agents arid essence . Many of these chemicals are known for causing allergies to the human tissue and hair. In the current invention we disclose a biotechnological shampoo preparation and its usage for efficient and ecological washing and maintenance of hair. Biotechnological shampoo is prepared with urease, bromelain along with other components. The preparation was efficient in removing dirt and odour without damaging the hair texture.