METHODE POUR INDUIRE LA DIFFERENCIATION D'UNE CELLULE MESODERMIQUE INTERMEDIAIRE EN UNE CELLULE PROGENITRICE RENALE, ET METHODE POUR INDUIRE LA DIFFERENCIATION D'UNE CELLULE SOUCHE PLURIPOTENTE EN UNE CELLULE PROGENITRICE RENALE
A method for producing renal progenitor cells from pluripotent stem cells, comprising steps (i) to (vi): (i) culturing the pluripotent stem cells in a culture medium containing FGF2, BMP4, a GSK-3ß inhibitor and retinoic acid or a derivative thereof； (ii) culturing cells produced in step (i) in a culture medium containing FGF2, a GSK-3ß inhibitor and BMP7； (iii) culturing cells produced in step (ii) in a culture medium containing FGF2, a GSK-3ß inhibitor, BMP7 and a TGFß inhibitor； (iv) culturing cells produced in step (iii) in a culture medium containing FGF2, a GSK-3ß inhibitor, BMP7, activin and a ROCK (Rho-kinase) inhibitor； (v) culturing cells produced in step (iv) in a culture medium containing retinoic acid or a derivative thereof and FGF9； and (vi) culturing cells produced in step (v) in a culture medium containing a GSK-3ß inhibitor and FGF9 to induce renal progenitor cells from intermediate mesodermal cells.
