#$%^&*AU2018101596A420181213.pdf#####ABSTRACT The present invention discloses a co-stimulator prepared by peptides from Hepatitis B virus(HBS) antigens and Hepatocellular Carcinoma antigens for antigen presentation (COST) and application thereof, and the COST comprises a peptide from HBV core antigens (HBcAg) located between amino acid of 18 and 27, peptides from HBV pre- S2 antigen located between amino acid of 44 and 53, and lysates from liver cancer cell line HepG2, wherein a mass ratio of the HBV core antigen (HBcAg) located between amino acid of 18 and 27, the peptides from HBV pre-S2 antigen located between amino acid of 44 and 53 and the lysates from liver cancer cell line HepG2 is 0.5~1:0.5~1:1~2. The DCs induced by the COST become a DC vaccine with a multiplex specific co-stimulator prepared by peptides from HBV-associated HCC, which can activate the proliferation and function of T cells, to enhance the function for fighting HBV-HCC. (Figure 5)30000 -- *---Normal liver Matched tissues -_-- HCC --- PBMC 20000 1000 0 - 1 0 0 10 100 1000 10000 25000 50000 100000 No. of Dendritic cells Figure 5 3/3
