A cell separation apparatus which do not damage a cell sample, prevent the exhaustion of an electrode to which an electric voltage is applied in the separation of a cell, and does not cause the clogging of channels over a long period of term for cell separation and is provided. The cell separation apparatus of the present invention comprises a means for moving a cell within a cell separating space by applying an external force to cells in the cell separating space from outside, and channels for separating and discharging the cell and which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis. As a means for applying an external force and a gel electrode containing electrolyte is preferred to apply an electric voltage to a cell separating space. The cell separation apparatus may further comprise a filter disposed at upstream of a channel through which a sample to be introduced into a cell separating space is passed and whereby impurities can be captured so that the clogging of channels is prevented.
