ЭМПРЕЗА БРАЗИЛЕЙРА ДЕ ПЕСКВИЗА АГРОПЕКУАРИА-ЭМБРАПА (BR)
发明人:
КАРВАЛЬО Клаудио Гильерми Портела Де (BR),ТОЛЕДО Жозе Франсиску Феррас Де (BR)
申请号：
RU2012112597/10
公开号：
RU2012112597A
申请日:
2009.08.31
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. A method of producing female inbred lines from hybrids of the family, in which the production of these lines consists of converting RHA inbred lines obtained by self-pollination of hybrids (commercial or non-commercial) into inbred HA lines for the modification of these HA lines into plants with male sterility, where indicated the method includes the following steps: a) crossing any inbred HA line (non-recurrent line) previously subjected to emasculation (or subjected to preliminary sterilization of pollen using chemical agents or other mechanisms), with the RHA inbred line (recurrence line) carrying the fertility reducing gene； b) obtaining F1 that carries 50% of the recurrence line genotype from stage (a)； c) backcrossing F1, previously subjected to emasculation (or subjected to pre-sterilization of pollen using chemical agents or other mechanisms), with the RHA recurrence line shown in (a)； d) obtaining backcross 1 (BC1) from the bearing 75% phenotype of the recurrence line indicated in (a)； e) identification of heterozygotes (Rfrf) and homo igot (RfRf) in the BC1 generation obtained in step (d)； f) backcrossing of heterozygous (Rfrf) and homozygous (RfRf) BC1 plants, previously subjected to emasculation (or subjected to preliminary sterilization of pollen using chemical agents or other mechanisms), with with the recurrent parent specified in (a), with obtaining BC2, only heterozygous plants of BC1 seeds were taken for the next offspring； g) obtaining generation BC from generation BC in accordance with stages (e) and (f)； h) identification and self-pollination of heterozygotes (Rfrf) and homozygotes (RfRf) n1. Способ получения женских инбредных линий из гибридов семейства, в котором получение этих линий заключается в преобразовании инбредных линий RHA, получаемых при самоопылении гибридов (коммерческих или некоммерческих), в инбредные линии НА для модифицикации этих линий НА в растения с мужской стерильностью, где указанный способ включает следующие стадии:a)
