A herbal formulation to ameliorate Fluoride toxicity in animals was developed by mixing Tamarind (Tamarindus indica) fruit pulp, drum stick (Moringa oleifera) unripe pods and whole oat grain powder in the ratio of 20:4:1 and molasses was used as vehicle to make it into pallet. The product was tested in experimental condition for its potential to reduce body fluoride burden by estimating fluoride concentration in serum, urine and bone. The safety of the formulation was ascertained after scientific validation of acute and chronic toxicity in rat model. There after the toxic effect of this formulation was studied in healthy calves @ 200mgl kg b.wt, daily for 90 days. Various parameters such as haematological changes, vital organ functions like heart, liver, kidney etc were evaluated. No evidence of toxicity could be detected at dosage 200 mg/kg orally for 90 days. There after the product was tried for its ameliorative potential in the field clinical cases of fluorosis in cattle and buffalo at the dose rate of 200 mg / Kg b. wt daily for 90 days. A total of Forty eight cattle and buffaloes having visible clinical lesions and elevated fluoride levels in serum (10.40ppm) and urine ((26.0 ppm) were selected for this study. The product significantly (p<；0.001) reduced serum fluoride burden and increased excretion of Fluoride in urine. There was significant improvement of general health, production and performance of animals of treatment group. No adverse effect of the formulation could be detected during 3 months observation period. The cross validation studies of the formulation were performed at two fluoride endemic areas of Rajasthan viz. Singpur and Chittaurgargh areas of Rajasthan. The cross validation report of this product documented 58.81% reduction of serum fluoride concentration in cattle and buffaloes at day 90 post treatment along with marked improvement in clinical score and other parameters. More ever, the administration of this herbal product to cattle and
