The present invention relates to a buffy coat extracting kit which is used to extract a buffy coat by collecting blood for an autologous blood skin regeneration procedure and a cell regeneration procedure. The buffy coat extracting kit comprises: a plasma unit (40), of a cylindrical shape which has a large diameter, which is produced in a transparent resin and forms a plasma layer when centrifuging blood； a buffy coat unit (70), of a tube shape which has a smaller diameter, which gradually changes the lower part of the plasma unit (40) and forms a buffy coat layer； a red blood cell unit (80) which forms one or more pairs of protrusions or a vertical or horizontal support rib (60) reaching the inner lateral side of a centrifuge bucket in the outer lateral side of the buffy coat unit (70) or the plasma unit (40) and forms a red blood cell layer which is a part of being directly inserted into the bucket of the centrifuge by being connected to the lower part of the buffy coat unit (70)； a lower packing (90) of an elastic body which seals the lower part of a kit main body (10) by being formed in the lower part of the red blood cell unit (80)； a lower stopper (100) of a structure which has an open center； and an upper stopper (20) which forms a screw line in the inner lateral side of the lower stopper (100), forms a screw line in the outer circumference side of a push stick (110) which corresponds to the same, prevents blood from being leaked by being combined with the upper end of the plasma unit (40) of the kit main body (10), is combined with an upper packing (30), injects blood and extracts a buffy coat by using a syringe, and has an open center.본 발명은 자가혈 피부재생 시술 및 세포재생시술을 위하여 혈액을 채혈하여 버피코트를 추출하는데 사용되는 버피코트추출키트에 관한 것으로, 투명한 수지로 제작되고, 혈액을 원심분리 했을 때 혈장 층이 형성되는 곳으로서 직경이 큰 원통 형상의 혈장부(40), 상기 혈장부(40)의 하부에 직경이 점진적으로 변하도록 하여, 버피코트 층이 형성되는 내경이 작은 대롱 형태의 버피코트부(70), 상기 혈장부(40) 또는 버피코트부(70)의 외측면에 원심분리기 버킷의 내 측면에 이르는 수직 또는 수평의 지지리브(60) 또는 한 쌍 이상의 돌기를 형성하고, 상기 버피코트부(70)의 하부와 연통되어,
