Being construction manner of the nervous spheroid network which is concatenated with the neurite which spheroid 2 or more including the neurocyte expanded from each spheroid, approaching, the manner which includes the process which cultures the said spheroid 2 or more it is laid out.神経細胞を含むスフェロイド2個以上が各スフェロイドから伸長した神経突起で連結された神経スフェロイドネットワークの構築方法であって、近接して配置された2個以上の該スフェロイドを培養する工程を含む方法。