Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) (RU)
发明人:
Нуколова Наталия Владимировна (RU),Алексашкин Антон Дмитриевич (RU),Клячко Наталья Львовна (RU),Кабанов Александр Викторович (RU)
申请号：
RU2013153079/15
公开号：
RU2013153079A
申请日:
2013.11.29
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. A polymer composition nanoparticle enzyme / polycation / polyanion comprising an antioxidant enzyme superoxide dismutase, characterized in that the enzyme is coated with an inner coating of a polycation and a polyanion of the outer shell, wherein the shell covalently crosslinked with each other or inside via a linker, wherein the nanoparticle It has a hydrodynamic diameter in the range 40-80 nm.2. A nanoparticle according to claim 1, characterized in that used as the polycation protamine or poliarginin.3. A nanoparticle according to claim 1, characterized in that it is used as a polyanion a poly (glutamic acid) -polietilenglikol.4. A nanoparticle according to claim 1, characterized in that it is used as a linker glutaraldehyde (GA) .5. A nanoparticle according to claim 1, characterized in that the linker used 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDC) .6. A process for preparing a dispersion containing polymeric nanoparticles of the composition of enzyme / polycation / polyanion complex according to claim 1, comprising an antioxidant enzyme superoxide dismutase, characterized in that to a solution of superoxide dismutase (SOD) was added dropwise with stirring polycation solution, then the mixture was stirred at room temperature for 30 min, after which was added dropwise with stirring a solution of polyanion, and the mixture was incubated at 4 ° C for 30 minutes, then dropwise added with stirring a solution lin EPA, the reaction mixture was allowed to stand for 8-12 hours at 4 ° C, after which the by-products and unreacted reactants are removed by repeated centrifugation using a filter system with a transmission limit of the ultrafiltration1. Полимерная наночастица состава фермент/поликатион/полианион, содержащая антиоксидантный фермент супероксиддисмутазу, характеризующаяся тем, что фермент покрыт внутренней оболочкой из поликатиона и внешней оболочкой из полианиона, при этом оболочки ковалентно сшиты друг с другом или внутри себя с помощью линкера, пр
