A physiological detecting device includes a measuring unit having a test strip end, a test strip reaction zone and a connecting end. The test strip end includes at least one test strip for taking a sample of a test solution containing at least one physiological hormone. The test strip reaction zone reacts with at least one physiological hormone detected on the test strip end. The engaging portion is engaged to the connecting end of the measuring unit in the first instance, and is also engaged to the test strip end of the measuring unit in the second instance whereby, in the second instance, the engaging portion is engaged to the test strip end of the measuring unit, so as to push the test solution sampled on the test strip end to the test strip reaction zone for the reaction of the test strip to be performed.
