Disclosed is a skin antiseptic agent which hardly causes a skin disorder such as rough skin and has a prolonged antiseptic effect. Also disclosed is a skin disinfection method which hardly causes a skin disorder such as rough skin and enables a prolonged antiseptic effect. The skin antiseptic agent comprises an N-acylated form of hydroxyproline or a salt thereof. A preferred example of the N-acylated form of hydroxyproline or the salt thereof is N-acetylhydroxyproline. Preferred examples of the antiseptic component include alcohols such as ethanol and isopropyl alcohol. The skin disinfection method may comprise applying or spraying a composition comprising an N-acylated form of hydroxyproline or a salt thereof onto the skin. The composition is preferably the skin antiseptic agent.
