A phantom for measuring thickness of a thin layer and a method of using thereof. The phantom may include a non-scattering muscle mimicking material having a flat top surface a plurality of soft tissue mimicking thin layers placed in a first area, which is at least a part of a top surface of the non-scattering muscle mimicking material, and having thicknesses different from each other and an anechoic blood mimicking liquid material placed in an area other than the first area among an entire area of the top surface of the non-scattering muscle mimicking material and on a top surface of the plurality of soft tissue mimicking thin layers.
