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Improved procedure for the preparation of Ginkgo extracts
专利权人:
Dr. Willmar Schwabe GmbH & Co. KG
发明人:
WAIMER, Frank,REINHARD, Steffen,HAUER, Hermann
申请号:
ES15700546
公开号:
ES2653446T3
申请日:
2015.01.12
申请国别(地区):
ES
年份:
2018
代理人:
摘要:
Procedure for the preparation of a dry extract from Ginkgo biloba leaves with a content of 22.0% by weight to 27.0% by weight of flavonoids, calculated as flavonglycoside, from 2.6% by weight to 3.2 % by weight of bilobálido, from 2.8% by weight to 3.4% by weight of ginkgolides A, B and C as well as a maximum of 5 ppm of ginkgolic acids, comprising the following steps: (a) extraction of fresh green leaves or dried Ginkgo biloba with aqueous acetone, an aqueous alkanol with 1 to 3 C atoms or anhydrous methanol at a temperature of about 40 to 100 ° C, to obtain a crude extract solution, (b) distillation separation from most of the acetone or alkanol with 1 to 3 C atoms of the crude extract solution of step (a) up to a maximum content of 10% by weight, where in the case of the use of anhydrous methanol in the stage (a), in the last stages of distillation water is added to obtain a concentrated aqueous solution , (c) dilution with water of the concentrated aqueous solution of step (b) to a solids content of 5 to 25% by weight, cooling to a temperature below 25 ° C, cold maintenance until the formation of a precipitate and separation of the precipitate arisen to obtain again an aqueous solution, (d) addition of ammonium sulfate to the aqueous solution obtained from step (c) and extraction of the solution that arises containing ammonium sulfate, with methyl ethyl ketone or a mixture of methyl ethyl ketone and acetone, separation of the aqueous phase from the methyl ethyl ketone or methyl ethyl ketone-acetone phase, to obtain a methyl ethyl ketone or methyl ethyl ketone-acetone phase, (e) concentration of the methyl ethyl ketone or methyl ethyl ketone phase from step (d) to a dry extract fraction of 40 to 80% by weight, to obtain a concentrate, (f) adjusting a fraction of 10 to 60% by weight of the concentrate of step (e) with water and methyl ethyl ketone to a fr dry extract action of a maximum of 60% by weight and a methyl ethyl ketone content of a maximum of 30% by weig
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