THE REGENTS OF THE UNIVERSITY OF COLORADO; A BODY CORPORATE
发明人:
Brian Freed,Kirsten Anderson,Christina Roark,Jennifer Matsuda
申请号：
US16609175
公开号：
US20200199616A1
申请日:
2018.04.25
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Methods of preventing or treating rheumatoid arthritis (RA) in a subject by introducing the DRB1*04:01K71E mutation that is resistant to RA. The resistant allele is introduced into the subject having or at risk of developing RA, using a HLA CRISPR/Cas9 vector that targets codon 71 in the HLA allele DRB1*04:01, introducing a single A to G point mutation in codon 71 by homology directed repair to alter the lysine at position 71 of the expressed protein to glutamic acid. This modified allele is affected in the subject's hematopoietic stem cells, which are then expanded and transplanted back into the patient. This microgene therapy confers RA-resistance via an autologous transplant. The invention includes isolated nucleic acids, vectors, recombinant viruses, cells, and pharmaceutical compositions to modify the HLA DRB1*04:01 allele.
