您的位置: 首页 > 农业专利 > 详情页

METHOD FOR ISOLATION OF SOLUBLE POLYPEPTIDES
专利权人:
National Research Council of Canada
发明人:
Tanha, Jamshid
申请号:
AU2013204781
公开号:
AU2013204781A1
申请日:
2013.04.12
申请国别(地区):
AU
年份:
2013
代理人:
摘要:
#$%^&*AU2013204781A120130509.pdf#####Abstract Polypeptides with desirable biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human VHs and VLs, are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human VHs and VLs are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human VHs and VLs are also identified. The VHs and VLs identified may be used to create further libraries for identifying additional polypeptides. Further, the VHs and VLs may be subjected to DNA shuffling to select for improved biophysical properties.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

意 见 箱

匿名:登录

个人用户登录

找回密码

第三方账号登录

忘记密码

个人用户注册

必须为有效邮箱
6~16位数字与字母组合
6~16位数字与字母组合
请输入正确的手机号码

信息补充