vetor de expressão t7 de escherichia coli, vetores para a co-expressão e co-purificação de polipeptídeos recombinantes em/com proteínas carregadoras, uso de vetores de expressão na obtenção de complexos com múltiplos antígenos r imunomoduladores
T7 Escherichia coli expression vector, vectors for the co expression and co purification of recombinant polypeptides \/ proteins loaders, use of vectors for expression in obtaining complex with multiple antigens and immunomodulators.The present invention relates to a vector for expression of recombinant proteins, antigens, particles similar to the pathogen, and complex imunog\u00eanicos, said vector (here referred to as pmrka) being produced by modification of plasmids comprising the sequence gene promoter T7 D And E.Coli, and this modification is characterized mainly by the replacement of the ampicillin resistance gene for gene resistance to kanamycin and by insertion of the sequence pair (sequence of partition which determines the efficient segregation of plasmids in the daughter cells Duran T cell division).In addition are provided based on the plasmid expression vectors that comprise pmrka additionally at least one of the exossomo gene sequences of P. abyssi, vectors these here called pmrka - exo, pmrka ring and psumac.They are also provided in this invention the vectors comprising additionally gene sequences with immunomodulatory activity \/ imunorreguladora, preferably the vectors pmrka - Z - Z - exo and pmrka - Z - Z - ring. Other aspects of the invention include the method for the production of such vectors for the expression and uses of vectors obtained.vetor de expressão t7 de escherichia coli, vetores para a co- expressão e co-purificação de polipeptídeos recombinantes em/com proteínas carregadoras, uso de vetores de expressão na obtenção de complexos com múltiplos antígenos e imunomoduladores. a presente invenção se refere a um vetor para expressão de proteínas recombinantes, antígenos, particulas semelhantes a patógeno, e complexos imunogênicos, dito vetor (aqui denominado pmrka) sendo produzidos por modificação de plasmídeos compreendendo a sequência gênica do promotor t7 de e. coli, sendo essa modificação caracterizada principalmente pela substituição
