CELL & TISSUE SYSTEMS, INC.;LIFELINE SCIENTIFIC, INC.
发明人:
BROCKBANK, KELVIN G. M.
申请号:
CA2753291
公开号:
CA2753291C
申请日:
2010.02.23
申请国别(地区):
CA
年份:
2017
代理人:
摘要:
Method for preserving tissue including immersing the tissue in a solutionhaving a cryoprotectant concentration ofat least 75% by weight, a cooling step where the tissue is cooled to atemperature between the glass transition temperature of thesolution having a cryoprotectant concentration of at least 75% by weight and -20°C, a storage step where the tissue is stored at atemperature between the glass transition temperature of the solution and -2O°C, a rewarming step, where the tissue is warmed, anda washing step.