Disclosed is a process to produce a composition containing 5&rsquo；-ribonucleotides comprising: a) Subjecting a yeast to autolysis under conditions at which at least 50% of the RNA remains in a form degradable into 5&rsquo；-ribonucleotides； b) Subjecting the autolysate to solid/liquid separation and recovering the RNA-containing cell wall fraction and wherein at least 75% of the RNA remains associated with the cell wall fraction； and c) Converting the RNA in the recovered RNA-containing cell wall fraction into 5&rsquo；- ribonucleotides, whereby the solid/liquid separation in step b) is done at a pH of lower than 3.5 and higher than 1.0.
