ALTROGGE, Ludger,GLEISSNER, Timo,HEINZE, Andreas,HERMSMEIER, Sven
申请号：
EPEP2015/059152
公开号：
WO2015/165881A1
申请日:
2015.04.28
申请国别(地区):
EP
年份:
2015
代理人:
摘要：
The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method,wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.L'invention concerne un dispositif destiné à appliquer un champ électrique à une suspension de cellules, comprenant au moins une chambre qui comprend au moins un espace interne (40) destiné à contenir la suspension, l'espace interne (40) comprenant au moins deux segments (41, 42), chaque segment (41, 42) comprenant au moins une électrode (43, 44) et les électrodes voisines (43, 44) étant séparées les unes des autres par au moins un espace (47) qui est au moins en partie rempli d'un matériau isolant (46) et les bords des électrodes (43, 44) en regard à l'intérieur de l'espace interne (40) étant arrondis. Le fait d'arrondir les bords des électrodes en
