Wilhelmus Theodorus Johannes Maria Christiaan Hermens,Saskia Jacoba Petronella Haast,Dennis Johan Biesmans,Andrew Christian Bakker
申请号：
US16179648
公开号：
US10533188B2
申请日:
2018.11.02
申请国别(地区):
US
年份:
2020
代理人:
摘要：
The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.
