The present invention is directed to a preparation of an adjuvant system to achieve required level of humoral and cellular immune response against antigen of interest. The current invention provides an adjuvant system comprising an immunostimulating reconstituted influenza virosomes (IRIVs) and immunopotentiators. The current invention illustrates that an antigen is adsorbed or incorporated into IRIVs and further formulated with lipophilic adjuvant such as MPL or glucopyranosyl lipid adjuvant (synthetic analogue of MPL).本發明關於佐劑系統之製備,該佐劑系統能達到針對所欲抗原的體液及細胞免疫反應之所需水準。本發明提供包含免疫刺激性重構型流感病毒顆粒(IRIV)及免疫增強劑(immunopotentiator)之佐劑系統。本發明說明抗原被吸收或納入IRIV中且進一步與親脂性佐劑(諸如MPL)或吡喃葡萄糖基脂質佐劑(MPL之合成類似物)經調製。
