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СКОНСТРУИРОВАННЫЕ ПОСЛЕДОВАТЕЛЬНОСТИ ДЛЯ ОБЛЕГЧЕНИЯ ЭКСПРЕССИИ АНТИГЕНОВ В NEISSERIA И СПОСОБЫ ИХ ПРИМЕНЕНИЯ
专利权人:
ЧИЛДРЕНЗ ХОСПИТАЛ ЭНД РИСЁРЧ СЕНТЕР ОКЛЕНД (US)
发明人:
ПАХОН ФЕЙТ Роландо (US)
申请号:
RU2014112052/10
公开号:
RU2014112052A
申请日:
2012.08.30
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A promoter comprising a 5 portion of the natural porA promoter from N. meningitidis, comprising the sequence ATGGTT spacer moiety and 3 of the natural promoter porA from N. meningitidis, comprising the sequence TATAAT, wherein the spacer contains a sequence of the formula N-TTTCA-N, where N is X (T / A) (T / A) (T / A) (T / G) (C / G) (C / G) (C / G / A) (G / T) CXa Nyavlyaetsya XXX, where: Xprisutstvuet or absent, and when present, it is a T or A Xprisutstvuet or absent, and when present, that is A or C Xprisutstvuet or absent, and when present, it is a T or G Xprisutstvuet or absent, and when present, it is A or G iXprisutstvuet or absent, and when present, is the G, where the 5 portion, a spacer and a 3 portion operably linked for transcription to N. meningitidis.2. The promoter of claim. 1, wherein the spacer contains a sequence ATATGCCTCCTTTCATA.3. The promoter of claim. 1, wherein the spacer contains a sequence TATATGCCTCCTTTCATA.4. The promoter of claim. 1, wherein the spacer contains a sequence ATAATGCCTCCTTTCATA.5. The promoter of claim. 1, wherein the spacer contains a sequence ATATGCATCATTTCATA.6. The promoter of claim. 1, wherein the spacer contains a sequence TTTTGCGGGCTTTCATA.7. The promoter of claim. 1, wherein the spacer contains a sequence TTTTGCGGGCTTTCAGGG.8. The promoter of claim. 1, wherein the spacer contains a sequence TTTTGCGGGCTTTCAG.9. Promoter having the formula, from 5 to 3: TFB-XE-TFB ATGgde relates to a transcription factor binding sequence of the native promoter nmb1523 E refers to the sequence length of the extension 66 couples promoter nmb1523 natural bases aX relates to a spacer sequence of the native promoter nmb1523, situated between the TFB and E, where ca1. Промотор, содержащий:5′ часть природного промотора porA из N. meningitidis, содержащую последовательность ATGGTTспейсерную часть и3′ часть природного промотора porA из N. meningitidis, содержащую последовательность ТАТААТгде спейсер содержит последовательность формулы N-TT
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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