An improved method for preparing squalene from a squalene-containing composition, said method comprising the steps of (a) a purification distillation carried out at a temperature T1； (b) a denaturing distillation carried out at a temperature T2； wherein steps (a) and (b) may be performed in either order； T1 and T2 are sufficient to cause squalene to boil； T2>；T1； and T2>；200° C.
