DUBENSKY, THOMAS W., JR.,PORTNOY, DANIEL A.,LUCKETT, WILLIAM S., JR.,COOK, DAVID N.
申请号：
CA2551644
公开号：
CA2551644C
申请日:
2004.12.23
申请国别(地区):
CA
年份:
2014
代理人:
摘要：
The present invention provides recombinant nucleic acid molecules, expressioncassettes, and vectors useful for expression of polypeptides, includingheterologous polypeptides, such as antigens, in bacteria. Some of therecombinant nucleic acid molecules, expression cassettes and vectors comprisecodon-optimized sequences encoding the polypeptides and/or signal peptides.Some of the recombinant nucleic acid molecules, expression cassettes, andexpression vectors comprise sequences encoding non-Listerial and/or non-secA1signal peptides for secretion of the polypeptides. The invention also providesbacteria comprising the nucleic acid molecules, expression cassettes, andexpression vectors, as well as compositions such as vaccines comprising thebacteria. Methods of making and using the bacteria, recombinant nucleic acidmolecules, and expression cassettes are also provided.
