The present invention relates to a novel gene regulating the virulence of Cryptococcus neoformans, and a use thereof. According to the present invention, anti-Cryptococcal or anti-fungal drug candidate materials can be effectively screened for. In addition, the present invention relates to a method for screening for drug candidate materials, which can bring a synergistic effect by being co-administered with a commercially available anti-Cryptococcal drug or anti-fungal drug. Furthermore, provided is a pharmaceutical composition having an anti-Cryptococcal or anti-fungal effect by increasing or decreasing the expression of transcription factors. The present inventors have performed a large-scale virulence test by using insect and animal models so as to identity transcription factors, and have analyzed a complex correlation between the transcription factors and in vivo and in vitro phenotypes of pathogenicity.
