Killmer John L.,Arhancet Juan Pedro Humberto,McLaughlin Patrick D.
申请号：
US201616062097
公开号：
US2018368422(A1)
申请日:
2016.12.07
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like Particles (VLPs) suitable for insect control applications. Specifically, the disclosure provides a method for controlling target insects comprising, transforming a microbial host with a first DNA sequence comprising a gene encoding a bacteriophage capsid protein and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the microbial host to express the first and second DNA sequences, isolating virus-like-particles (VLPs) comprising the capsid protein and RNAi precursor from the microbial host, and contacting the isolated VLPs with the target insects.
