PURPOSE: A natural food preservative containing a glycyrrhizae radix extract, a paeonia suffruticosa andrews extract, and a coptis japonica extract are provided to offer the excellent antibiotic and anticancer effect. CONSTITUTION: A producing method of a natural food preservative comprises the following steps: separately mixing glycyrrhizae radix, paeonia suffruticosa andrews, and coptis japonica with alcohol, and heating the mixture to extract; filtering the obtained extracts before decompress-concentrating; freeze-drying the obtained concentrates; and mixing the obtained concentrates in the same concentration.본 발명의 실시예들은 감초 추출물, 목단피 추출물 및 황련 추출물을 유효성분으로 함유하는 식품 보존제를 제공한다. 상기 추출물들은 항균, 항산화 및 항암효능이 있어, 인체에 무해한 천연 식품 보존제로 사용할 수 있다.