Phytocidal active constitutent of glyphosate dimethylamine salt crystal, preparation method thereof, solid preparation containing the crystal and the use are disclosed. The preparation method is as follows: reacting glyphosate with dimethylamine gas at a molar ratio of 1:1.0-3.0 in organic solvent at 5~90℃ for 1.5~5 h, cooling slowly to -5~50℃, adding crystal seed for crystallization under stirring slowly for 1.0~2.0 h, filtering and drying.