A bolus assembly is configured to receive and be removably coupled to a transducer assembly of an ultrasound probe assembly. The bolus assembly includes a housing assembly and at least one sealing member. The housing assembly has a body having first and second ends, and a sidewall extending therebetween, the body forming a cavity defined by the first end, second end, and sidewall, the cavity being configured to receive at least one transducer of a transducer assembly, a first passage extending through the second end of the body, the sidewall including at least one opening spaced-apart from the passage and an acoustically transparent and distensible membrane attached to an entire outer periphery of the opening. The sealing member has a through hole and is positioned within the passage. The through hole is configured to receive the shaft. The sealing member is sealingly engaged with the shaft within the passage.