The present invention relates to the compositions and method of its preparation, manufacture and therapeutical uses, wherein, these compositions include one or more polynucleotides (for example, mRNA), encode the binding molecule (for example, antibody or its antigen-binding portion thereof) being specifically bound to. CTLA-4(cytotoxic T lymphocytes GAP-associated protein GAP 4). Treatment method disclosed herein includes, for example, application encodes one or more mRNA of anti-CTLA-4 antibody or its antigen-binding portion thereof with treating cancer in the subject of needs, such as the size by reducing tumour or the growth for inhibiting tumour. f.In some respects, the disclosed anti-CTLA-4 antibody of coding or the mRNA or mRNAs of its antigen-binding portion thereof are administered in tumor. When expressing disclosed anti-CTLA-4 antibody or its antigen-binding portion timesharing using multiple mRNA, the ratio of mRNA can be identical or different.
