The invention relates to a specific fraction of Aloe vera extract for enhancing tight junction in skin cells. The invention more particularly relates to use of such a fraction in skin, oral and hair care compositions. The inventive extract of Aloe vera comprises 60 to 95% by weight of polysaccharides having a molecular weight in the range of 2 to 3 kDa, and less than 5% polyphenols, wherein the polysaccharides are hydrolysed by trifiuoroacetic acid.