The present invention provides a royal jelly solution and a manufacturing method thereof. The method includes: mixing a royal jelly material with water into an aqueous royal jelly suspension, and adding an enzyme containing cysteine endonuclease into the aqueous royal jelly suspension for a reaction under a temperature of 20℃~70℃, so as to obtain a curdy royal jelly solution in a stable suspension state. By adding the enzyme containing cysteine endonuclease into the aqueous royal jelly suspension, the present invention may enhance the hydration capability of protein molecules in the royal jelly solution, such that the protein molecules in the royal jelly solution will not be easily precipitated by layers and can be in a stable suspension state. Thus, the present invention may preserve the whole components of royal jelly and have higher nutrition value without centrifugation, filtration, emulsification and homogenizing processing, and thus reduce the processing steps to achieve better processing efficiency and economic benefits.一種蜂王乳溶液及其製造方法,該方法包含:將一蜂王乳原料與水混合為一蜂王乳水懸浮液,於該蜂王乳水懸浮液中添加含有半胱胺酸內切酶的酵素後,使其在溫度20℃~70℃下進行反應,就能獲得一凝乳狀且呈穩定懸浮狀態的蜂王乳溶液。藉由在該蜂王乳水懸浮液中添加含有半胱胺酸內切酶的酵素,能夠提高該蜂王乳溶液中蛋白質分子的水合能力,使該蜂王乳溶液中的蛋白質分子不易分層沉澱且呈穩定懸浮狀態,因而不需離心、過濾、乳化與均質處理,藉此能保留蜂王乳全成分而有更高營養價值,並能減少處理程序而有較佳的製程效率與經濟效益。