It is validity that the present invention, which is by sugar enzyme extract, improves the manufacture object of wrinkle of skin including validity. The effect of manufacture object of the invention inhibits the aobvious of MMP -1 protein in the blood matter fibrocyte of the mankind, increases the generation of burning collagen type, prevents the generation of wrinkle of skin, improves damage wrinkle of skin. Comprehensive, manufacture object of the invention can prevent and improve wrinkle of skin.