The subject invention is based on unexpected more than additive effect of multiple applications of at least one cyclopropene on crop yield as compared to single applications. Provided are methods of increasing yield of a plant comprising contacting the plant with multiple applications of a cyclopropene. In one aspect, the method comprises (a) contacting the plant with a first composition comprising a cyclopropene; and (b) contacting the plant with a second composition comprising a cyclopropene; thereby increasing the yield of the plant in comparison to a plant not contacted with the first composition and/or the second composition. In another aspect, the method comprises contacting the plant with two or more separate applications of a composition comprising at least one cyclopropene thereby increasing the yield of the plant in comparison to a plant not treated or contacted with two or more separate applications of a composition comprising at least one cyclopropene.