This invention, operating the cell directly, hard offers the medicine which makes the promotion of histodifferentiation, the promotion of cell differentiation and the reinforcement of alkaline phosphatase activity of the cell cause. Concretely, the sulfuric acid basis is kept, at the same time, gurikosaminogurikan where the following (1) and (2) has quality or that salt is designated as the active ingredient, hard the histodifferentiation accelerator, the differentiation induction medicine of the cell and the alkaline phosphatase active reinforcement medicine of the cell are offered (1) repetition structure of two sugars which consist of the hekisuron acid residue and the glucosamine residue is designated as the basic framework, (2) the hirodokishiru basis of 2 rank of the hekisuron acid residue the description above (1) in the basic framework and the hydroxyl basis of 6 rank of the glucosamine residue or the inside either of the amino group of 2 rank of the glucosamine residue the sulfuric acid basis is not kept in the position of one or less. Furthermore as for the hekisuron acid residue, the glucuronic acid residue or those which is are the remaining overnight acid residue are desirable.本発明は、細胞に直接的に作用して、硬組織形成の促進、細胞分化の促進、細胞のアルカリフォスファターゼ活性の増強を惹起させる剤を提供する。具体的には、硫酸基を保持し、かつ、次の(1)及び(2)の特性を有するグリコサミノグリカン又はその塩を有効成分とする、硬組織形成促進剤、細胞の分化誘導剤及び細胞のアルカリフォスファターゼ活性増強剤を提供する;(1)ヘキスロン酸残基とグルコサミン残基とからなる二糖の繰り返し構造を基本骨格とする、(2)前記(1)の基本骨格におけるヘキスロン酸残基の2位のヒロドキシル基、グルコサミン残基の6位のヒドロキシル基及びグルコサミン残基の2位のアミノ基のうちいずれか1つ以下の位置には硫酸基を保持していない。なおヘキスロン酸残基は、グルクロン酸残基又はイズロン酸残基であるものが好ましい。
