VORONINA, Vera,MACDONALD, Lynn,ZAMBROWICZ, Brian,MURPHY, Andrew J.
申请号：
SG11201704646Y
公开号：
SG11201704646YA
申请日:
2015.12.18
申请国别(地区):
SG
年份:
2017
代理人:
摘要：
Methods and compositions are provided for making one or more targeted genetic modifications at a target genomic locus within a cell and for producing non-human animals comprising the modified genomic locus. The methods employ two or more large targeting vectors which are capable of recombining with each other and with the target genomic locus in a single genomic targeting step. The methods may also be employed in combination with a nuclease agent. Methods and compositions are also provided for enhancing homologous recombination at a target genomic locus in a cell. The methods employ two or more nucleic acids comprising one or more overlapping sequences. The methods may be employed in combination with a nuclease agent or without a nuclease agent.
