Methods and devices for delivering an active agent into the eye of a subject are provided. In one aspect, an ocular device for delivering an active agent through a cornea of an eye of a subject can include a housing configured to couple to the eye of the subject and a corneal seal coupled to the housing and positioned in the housing to encircle the cornea during use to form a corneal region. The device can also include an active agent reservoir coupled to the housing and positioned to release active agent into the corneal region, and a pressure regulator coupled to the housing and operable to introduce negative pressure between the housing and the eye. The corneal seal is operable to fluidically isolate the corneal region from the sclera in response to the negative pressure, thus minimizing contact of the active agent with the sclera.
