PURPOSE: PVA-Gel hydrogel according to the manufacturing method of hydro gel is provided to enhance the high potential bone reproducibility due to bio compatibility and mechanical characteristic. CONSTITUTION: A manufacturing method of cross-linking polyvinyl alcohol - gelatin hydrogel for the bone regeneration comprises the first step that manufactures 10 to 20 weight of gelatin solution by 10 to 20 weight% of polyvinyl alcohol water solution by putting the polyvinyl alcohols (weight-average molecular weight 89,000~98,000 g/mol, hydrolysis ~98%) which shows bio compatibility to heat it at 90°；C and mixing gelatin to the ultrapure water； the second step that synthesizes the cross-linked polyvinyl alcohol - gelatin hydrogel by inserting griffin as a crosslinking agent after mixing the polyvinyl alcohol solution and the gelatin solution on 50:50 v/v%； and the third step of freeze-drying this for two hours at -80°；C, freeze-drying for a day at -70°；C and drying for 24 hours at room temperature after molding the cross-linked polyvinyl alcohol - gelatin hydrogel in the synthetic resin mold.가교결합된 폴리비닐알콜-제라틴 하이드로겔은 가교결합제로서 제니핀이 사용된 것으로, 생체적합성 및 기계적인 특징 때문에 높은 잠재적 뼈 재생 가능성을 높여 준다. 본 발명의 하이드로젤은 생체의학공학, 의약, 그 밖에 다른 응용분야에서도 다양하게 이용될 것이다.
