The present invention provides a tissue regeneration substrate that has the ability to release a basic fibroblast growth factor (bFGF) and like cell growth factors in a sustained manner, into which cells can easily enter, and that is suitably used for regeneration of tissues. Specifically, the present invention provides a tissue regeneration substrate that comprises a cell growth factor adsorbed in a bioabsorbable porous substrate that contains collagen and gelatin, and a method for producing the same.