LEE, KUN FENG,李昆峰,李昆峰,CHEN, CHIEN AN,陈建安,陳建安,CHANG, HONG WEN,张鸿文,張鴻文,LIN, YUH JIUAN,林玉娟,林玉娟
申请号：
TW098117415
公开号：
TWI484186B
申请日:
2009.05.26
申请国别(地区):
TW
年份:
2015
代理人:
摘要：
The invention provides method for continuous detecting a glucose concentration in a sample, including: (a) providing a biosensor including a transducer and a polysaccharide over a surface of the transducer (b) providing a carbohydrate binding protein solution with a specific concentration, wherein the carbohydrate binding protein at least has one receptor, and the receptor has a binding ability to the polysaccharide and glucose (c) mixing a sample and the carbohydrate binding protein solution with the specific concentration to form a mixture (d) contacting the mixture with the biosensor (e) detecting an amount of the carbohydrate binding protein bound to the polysaccharide by the biosensor, wherein a glucose concentration of the sample is inversely proportional to the amount of the carbohydrate binding protein bound to the polysaccharide and (f) refreshing a surface of the biosensor with a high concentration glucose solution.本發明提供一種連續偵測待測樣本中葡萄糖濃度的方法,包括:(a)提供一生物感測器,其中該生物感測器包括一換能器與一多醣體覆蓋於該換能器之表面上;(b)提供一特定濃度之醣類接合蛋白溶液,其中該醣類接合蛋白具有至少一受器,且該受器具有與該多醣體及葡萄糖結合之能力;(c)將一待測樣本與該特定濃度之醣類接合蛋白溶液混合成一混合物;(d)將該混合物與該生物感測器接觸;(e)以該生物感測器偵測與該多醣體結合之該醣類接合蛋白的量,其中該待測樣本之葡萄糖濃度與該多醣體結合之醣類接合蛋白的量成反比關係;以及(f)以一高濃度葡萄糖溶液恢復該生物感測器之表面。100...生物感測器101...換能器(transducer)103...多醣體105...接合蛋白溶液107...醣類接合蛋白107a...全部受器為葡萄糖所佔據之醣類接合蛋白107b...部分受器為葡萄糖所佔據之醣類接合蛋白107c...未受葡萄糖佔據之醣類接合蛋白109...待測樣本111...葡萄糖113...混合物
