Disclosed is a method for preparing a cell growth scaffold having a structural memory feature, comprising a step of preparing a micro-fibrous or flocculent acellular tissue matrix material； a step of preparing an acidification-treated hydrogel-like acellular tissue matrix particles； proportionally mixing the prepared micro-fibrous or flocculent acellular tissue matrix material with the acidification-treated hydrogel-like acellular tissue matrix particles, followed by injection molding, freezing treatment, radiation treatment, and ultimately preparing a porous cell growth scaffold that can be stored at room temperature. The cell growth scaffold prepared from this method is a porous cell growth scaffold that has no chemical crosslinking, and has a biological activity, a stable three dimensional structure and a structural memory feature. The cell growth scaffold has an excellent biocompatibility and complete biodegradability, and supports the growth of cells and the growth of tissues and organs in vitro and in vivo, thereby being suitable for repair of human soft tissue traumas and defects.
